Efficient isolation and mapping of rad genes of the fungus Coprinus cinereus using chromosome-specific libraries
نویسندگان
چکیده
منابع مشابه
Efficient isolation and mapping of rad genes of the fungus Coprinus cinereus using chromosome-specific libraries.
We have constructed cosmid libraries from electrophoretically separated chromosomes of the basidiomycete Coprinus cinereus. These libraries greatly facilitate the isolation of genes by complementation of mutant phenotypes and are particularly useful for map-based cloning strategies. From a library constructed from two co-migrating C.cinereus chromosomes, we isolated a clone that complements the...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1992
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/20.15.3993